Basic Info.Model NO.WIX-fastBLOTWarranty1
YearCustomizedCustomizedStructureDesktopMaterialPlasticCertificationCEApplicationLabTypeWestern
BlotOEMYesODMYesBlotFast Semi-DryTimeMax 7
MinsTrademarkWIXTransport PackageCarton/PalletOriginChinaProduct
DescriptionWIX-fastBLOT Fast Semi-dry Blot
System Product Description1. Working condition1.1
Temperature: 5ºC~40ºC; Relative moisture: 20%~80%2.
Technical requirement2.1*Blot throughout: 4 small gels or 2
medium gels; 2 blot cassettes, one of the two cassettes can be
used for operation or two cassettes can be used respectively in the
right orders. Two independent blot programme can be used at the
same time. The blot area is 200*100mm*2.2.2 The world uniquely
design for high efficient heat dissipation channel, is to
release the heat during the operation and ensure the
reliability of the lab in the low temperature.2.3
Easy operation: optional ready-to-use blot consumable
package, no need to prepare buffer and membrane;2.5 *Blot
speed: blotting 2 small gels within 3 minutes, blotting 4
small gels or 2 medium gels within 7 minutes.2.5
*Power: Built-in integrated power supply, voltage
0-30V (1V adjustable) current (0-3A) (0.1 adjustable).2.6
Customer interface, 5-inch capacitive touch screen, sensitive
touching screen, convenient operation. Automatic programme running;
The preset programme guides the lab design and modifies the
programme manually on the basic of the needs (9 groups of data
are available for recorded automatic storage and customer-decisive
storage).2.7 Electrode Design: Buckle locking mode with plate
electrode design ensure uniformity of pressure and field
strength.2.8 Application: Being compatible with traditional
lab reagent and consumable.2.9
Dimension (mm): 290x218x2003.0 Weight: 3.8kg3.
Configuration requirement1 set of Blot System (including built-in
power supply and 2 pieces of blot cassettes)Detailed
Photos CertificationsPackaging & ShippingInstallation
InstructionsExperiment preparationWix-fastBLOT Fast Semi-dry
Blot System, fast transfer filter papers (5
sheets as 1 unit), gel, NC membrane or PVDF
membrane (need to be activated with methanol before transfer),
and roller. Experiment StepsPlace 1 unit of fast transfer
filter papers (including 5 sheets of fast transfer
filter papers) in the center of the cassette base. If transfer 1
mini gel, place the gel sandwich in the center of the cassette
base, if transfer 2 mini gels, place 2 gels at two sides of the
cassette base.Blotting buffer: Methanol: deionized water = 1:1:3
(V/V) ratio for diluting blotting buffer. Immerse fast transfer
filter papers with blotting buffer until it is completely wetted
(about 15 ml).NC or PVDF membrane is placed on top of the fast
transfer filter papers, and use roller to remove any air
bubbles.Carefully align the gel on the membrane. If necessary,
gently use roller to remove air bubbles between gel and
membrane.Place the other unit fast transfer filter papers
(including 5 sheets of fast transfer filter papers) on
top of the gel, immerse gel switch with blotting buffer to make it
completely wetted (about 15 ml), use roller to remove any air
bubbles.Put cassette lid on the base, ensure the red side of the
lid and handle of the cassette base be aligned vertically,
carefully press vertically and check to ensure the electrical
contacts fit closely into slots in the base.Slide cassette into one
the of fast blot bays until it makes contact with the magnetic
interlock in the back of the instrument tub and you hear a
click.Set transfer conditions according to below data. WIX-fastBLOT
has two modes, one is A=B, set protocols of the two cassettes are
the same, the other is A≠B, set protocols of the two cassettes are
different.Molecular weight10-90kDa90-190kDa>190kDa1 mini gel per
cassetteconstant current 1.3A 10 minconstant current 1.3A15
minconstant current 1.3A17 min2 mini gels per cassetteconstant
current 2.6A 13 minconstant current 2.6A17 minconstant current
2.6A19 minTake out gel after blotting.Caution: Put cassette lid on
the base, carefully press vertically and check to ensure the
electrical contacts fit closely into slots in the base.If only 2
mini gels need to be transferred, A=B mode is recommended, 1 gel
per cassette, under such conditions, generated heat by each
cassette is a little so that to achieve a better experiment result.
Put NC or PVDF membrane into block buffer immediately after it is
taking out from the cassette, in order to avoid dried membrane
leading by transfer liquid volatilization.If transfer time is
longer that much heat generated, put NC or PVDF membrane into bock
buffer immediately after it is taking out from the cassette, to
avoid dried membrane.Company ProfileWIX TECHNOLOGY BEIJING CO., LTD
(WIX for short) was established in 2015. It is a technology-based
private manufacturing enterprise integrating product development,
manufacturing, marketing, and technology consulting. WIX mainly
develops and produces instruments in the field of life scienc
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